23 August 2022: Image Quiz #89

Authors: Ruhani Sardana, M.B.B.S.Anil Parwani, M.D., Ph.D., M.B.A.

Which technique is shown in the image below?

Select an answer:

A. Electron microscopy

B. Enzyme linked immunosorbent assay

C. Fluorescent in situ hybridization

D. Immunofluorescence

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23 June 2021: Pathology Images of the Week

Here is a selection of new images from our free, online textbook:

Informatics, digital & computational pathology > Data representation and communication standards

The PNG image has been encoded into JPG using a high quality setting (approximately 329 KB).

Contributed by Chris Williams, M.D.

Uterus > Low grade endometrial stromal sarcoma

Deceptively well circumscribed tumor with a tan-yellow soft cut surface; microscopic evaluation revealed myometrial and lymphovascular invasion.

Contributed by Elizabeth Kertowidjojo, M.D., Ph.D., M.P.H.

Colon > Ulcerative colitis

Active chronic colitis with cryptitis is noted.

Contributed by Rachel Horton, D.O. and Catherine E. Hagen, M.D.

Liver & intrahepatic bile ducts > Histology

Periodic acid-Schiff with diastase (PASD) highlights the basement membrane of bile ducts. It also stains alpha-1 antitrypsin globules in hepatocytes in the setting of alpha-1 antitrypsin deficiency. The lack of globules in this image is a normal finding.

Contributed by Kimberley J. Evason, M.D., Ph.D.

Ovary > Yolk sac tumor

Schiller-Duval body

Contributed by Sharon Song, M.D.

16 June 2021: Pathology Images of the Week

Here is a selection of new images from our free, online textbook:

Laboratory Administration & Management of Pathology Practices > Lean workflow / Six Sigma

An organized workspace with a space for all needed tools or materials for jobs done at the station readily allows one to identify when something is missing or out of place.

Contributed by Stephen J. Hardy, M.B.A.

Liver & intrahepatic bile ducts > Ductular reaction

The ductular profiles with complete and incomplete lumina at the portal – parenchymal interface are highlighted by CK19.

Contributed by Shahbaz Khan, M.D.

Skin nonmelanocytic tumor > Juvenile xanthogranuloma

Right leg, excision, juvenile xanthogranuloma, scattered Touton giant cells.

Contributed by Lamiaa Hamie, M.D., M.Sc. and Ossama Abbas, M.D.

Bladder, ureter & renal pelvis > Noninvasive papillary urothelial carcinoma high grade

High power showing architectural disorder, loss of polarity and nuclear pleomorphism in high grade papillary carcinoma.

Contributed by Michelle R. Downes, M.D.

Informatics, digital & computational pathology > Fluorescent microscopy applications

Interphase FISH showing translocation involving the EWSR1 gene at 22q12.

Contributed by Ruhani Sardana, M.B.B.S. and Anil Parwani, M.D., Ph.D., M.B.A.

2 December 2020: Images of the Week

Here are just a few of this week’s new images from our free, online textbook:

Low grade intraosseous (central) osteosarcoma

Cellular fascicles of spindle cells with mild nuclear atypia in a fibrosclerotic stroma.

Contributed by Borislav A. Alexiev, M.D.

AML with inv(3)(q21.3;q26.2) or t(3;3)(q21.3;q26.2)

Bone marrow aspirate showing a hypolobated megakaryocyte, increased blasts and a pseudo-Pelger-Hüet neutrophil.

Contributed by Alexa J. Siddon, M.D.


Snapshot of dynamic microscopy from the pathologist end (10x objective), showing annotations and the control buttons.

Contributed by Jason A. Thomas, C.T. and Sigfred Lajara, M.D.

Liver & intrahepatic bile ducts → Viral hepatitis → COVID-19

Occasional cases (15%) have sinusoidal microthrombi (600x).

Contributed by Stephen M. Lagana, M.D.

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